platelet derived growth factor receptor beta  (R&D Systems)

Journal: Cancer Research

Article Title: Discovery and Evaluation of Biomarkers for Triple-Negative Breast Cancer Subtypes Uncovers Patient Stratification and Targeted Therapeutic Strategies

doi: 10.1158/0008-5472.CAN-24-2758

Figure Lengend Snippet: TAGL as a predictive marker for dasatinib sensitivity. A, Western blot of control and TAGLN -KO cells in Hs578T (top) and BT-549 (bottom) cells. B, Representative immunofluorescence of Hs578T and BT549 control and TAGLN -KO cells, stained with αTAGL (green) and DAPI (blue). Scale bar, 50 μm. C, Cell proliferation in control (black) and TAGLN -KO (purple) cells measured by MTT assays over 72 hours. D, Relative percentage of migrated cells in control and TAGLN -KO cells, assessed by transwell assays. In C and D , values represent the mean ± SEM of at least three experimental replicates, and statistical significance was determined using one-way ANOVA. E, Drug–response curves for cell viability of control and TAGLN -KO cells treated with dasatinib at increasing concentrations. F, Western blot of known dasatinib targets in control and TAGLN -KO cells. G, Data from the DepMap portal showing the correlation between TAGLN and PDGFRB in breast cancer cell lines. H, Western blot of PDGFRβ in TAGLN -KO and cells and clones constitutively expressing PDGFRB . I, Drug–response curves for cell viability of TAGLN -KO and TAGLN -KO/ PBGFRB cells treated with dasatinib at increasing concentrations. In E and I , solid lines represent the mean of three biological replicates performed in technical replicates. The dashed line indicates their IC 50 value. **, P < 0.01; ****, P < 0.0001; ns, not significant.

Article Snippet: For PDGFRB overexpression, lentiviral particles were generated using the PDGFRB Human Tagged Lenti ORF Clone (RC206377L4, OriGene) plasmid and used to transduce target cells.

Techniques: Marker, Western Blot, Control, Immunofluorescence, Staining, Clone Assay, Expressing

Journal: bioRxiv

Article Title: Estrogen receptor-positive cell line xenograft models recapitulate metastatic dissemination and endocrine response of invasive lobular breast carcinoma

doi: 10.64898/2026.03.17.712396

Figure Lengend Snippet: (A-B) Masson’s trichrome (left) and PDGFRβ (right) staining of primary tumors from MDA-MB-134 (A) and SUM44PE (B) orthotopic xenografts and spontaneous metastases to the indicated organs. (C) Masson’s trichrome staining of primary tumors from MDA-MB-330 orthotopic xenografts and spontaneous metastases to the indicated organs. (D) Ly6G staining of primary tumors from MDA-MB-134, SUM44PE, MDA-MB-330 and BCK4 orthotopic xenografts. Insets show a small portion of the images at higher magnification. Scale bar: 40 μm.

Article Snippet: Primary antibodies were as follows: ERα (clone SP1 from Roche, #05278414001), CDH1 (Clone 36 from Roche, #05905290001), p120 (clone 98/pp120, BD Biosciences, BD610134), PR (clone 1E2, Roche, 05278392001), PDGFR (Cell Signaling Technologies, CST #3169, 1:100), and Ly6G (BD, #551459, 1:100).

Techniques: Staining